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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brain

Fig. 8

STAT1α and STAT1β isoforms in the hippocampus are phosphorylated in the absence of IFNγ during a viral infection. Lysates of hippocampal explants from CD46+ (ag) and CD46+/IFNγ-KO (hn) control and MV-infected mice were analyzed by western blot for phosphorylated (activated) STAT1 (b, c, i, j), and total STAT1 (d, e, k, l) proteins. GAPDH was used as a protein loading control. Representative blots are shown in a and b. Total levels of STAT1α (upper band; d, k) and STAT1β (lower band; e, l) were significantly increased in MV-infected hippocampal explants from CD46+ pups (d, e) at 7 and 10 dpi and in CD46+/IFNγ-KO pups (k, l) at 10 dpi. Phosphorylation of STAT1α (STAT1α-P; B, I) increased significantly in CD46+ explants at 7 and 10 dpi (b) and CD46+/IFNγ-KO explants at 10 dpi (I). Phosphorylation of STAT1β (c, j) was increased in CD46+ explants at 7 dpi only (c) and in CD46+/IFNγ-KO explants and 7 and 10 dpi (j). Protein ratios of STAT1α-P/STAT1α showed increased activation of phosphorylation of STAT1α-P at 10 dpi in CD46+ mice (f), but no activation in CD46+/IFNγ-KO mice (m). The protein ratios of STAT1β-P/STAT1β showed decreased activation of phosphorylation during infection in CD46+/IFNγ-KO mice (n), but not in CD46+ mice (g). Statistical analysis was applied by one-way ANOVA with multiple comparisons. (**p < 0.01, ***p < 0.001, ****p < 0.0001 significantly different uninfected versus MV-infected; n = 4)

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