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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brain

Fig. 9

STAT2, but not STAT3, is activated during MV infection in the neonatal hippocampus. Lysates of hippocampal explants from CD46+ (black bars; b, d, f, I, k, m) and CD46+/IFNγ-KO (grey bars; c, e, g, j, l, n) control and MV-infected mice were analyzed by western blot for phosphorylated (activated) STAT2 (STAT2-P; b, c), total STAT2 (d, e), STAT2-P/STAT2 (f, g), STAT3-P (i, j), total STAT3 (k, l), and STAT3-P/STAT3 (m, n). GAPDH was used as a protein loading control. Representative blots are shown for STAT2 (a) and STAT3 (h). STAT2-P levels were significantly increased in MV-infected hippocampal explants in CD46+ (b) and CD46+/IFNγ-KO (c) pups at 7 dpi, though STAT2 phosphorylation was sustained to 10 dpi in CD46+ explants. Protein ratios of STAT2-P/STAT2 showed significantly increased activation of phosphorylation in CD46+ mice at 3 and 7 dpi (f). STAT3 phosphorylation was not significantly induced under any condition (g, h, m, n). Levels of total STAT3 protein were increased with infection at 10 dpi in CD46+ explants (i), but not in CD46+/IFNγ-KO explants (j). Statistical analysis was applied by one-way ANOVA with multiple comparisons (*p < 0.05, ***p < 0.001, ****p < 0.0001 significantly different uninfected versus MV-infected; n = 4)

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