Fig. 1

Potent effects of CNO and NDC in protecting DA neurons against LPS-induced neurotoxicity. Rat mesencephalic neuron-glia cultures were pretreated with vehicle or indicated concentrations of CNO, NDC, or clozapine for 30 min before the addition of LPS (15 ng/ml). Seven days after LPS treatment, the protective effects of CNO, NDC, or clozapine against inflammation-elicited damage of DA neurons were determined by quantifying functional changes of [³H]DA uptake capacity (a, b, and f) and counts of THir neurons (c, d). E Representative images of anti-TH immunostaining in cultures were shown. Results are expressed as the mean ± SEM or as a percentage of vehicle controls (mean ± SEM) from five independent experiments performed in triplicate. **p < 0.01, ^# p < 0.05, ^## p < 0.01. Scale bar = 50 μm