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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Clozapine metabolites protect dopaminergic neurons through inhibition of microglial NADPH oxidase

Fig. 6

CNO and NDC inhibit NOX2 activation. a HAPI microglia cells were pretreated with CNO (0.01 μM)/NDC (0.1 μM) for 30 min followed by LPS stimulation. Fifteen minutes later, cells were harvested. The levels of p47phox were determined in both membrane and cytosolic fractions using Western blot. GAPDH and gp91phox were used for internal controls of cytosolic and membrane fractions, respectively. b The band density of blots were quantified. Results are expressed as a percentage of PMA group or vehicle controls (mean ± SEM) from three independent experiments performed in triplicate. **p < 0.01, # p < 0.05, ## p < 0.01

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