Fig. 7

CNO and clozapine are equipotent in attenuating MPTP-induced dopaminergic neuron damage and motor deficits but show differential effects on blood WBC counts. a Experimental designs. Eight-week-old male C57BL/6J mice received daily MPTP injections (20 mg/kg, s.c.) for six consecutive days. From 1 day prior to MPTP injection, clozapine or CNO (1 mg/kg, s.c.) was administered twice daily for 21 consecutive days. b Fourteen days after initial MPTP injection, the protective effects of clozapine and CNO against MPTP-induced motor deficits were measured by the rotarod test. c Twenty-one days after the first injection of MPTP, dopaminergic neurons in the SNpc were immunostained with anti-TH antibody and the numbers of THir cells in SNpc were counted. d The representative images of TH staining in the SN were shown. e Blood WBC counts were shown in clozapine- and CNO-treated mice. Results are expressed as the mean ± SEM or as a percentage of vehicle controls (mean ± SEM). **p < 0.01, ^# p < 0.05, ^## p < 0.01; n = 4–8. Scale bar = 100 μm