Fig. 8
From: Clozapine metabolites protect dopaminergic neurons through inhibition of microglial NADPH oxidase
CNO and clozapine attenuate MPTP-induced reactive microgliosis with equi-potency. a Eight-week-old male C57BL/6J mice started to receive 1 mg/kg clozapine or CNO (s.c., twice daily) 1 day before MPTP injection (20 mg/kg, subcutaneously for six consecutive days). After 8 days of initial MPTP injection, microglia in the SN were stained with anti-CD11b antibody and the representative images were shown. b The inhibitory effects of clozapine or CNO on microglial activation were determined by analyzing the density of CD11b immunostaining. Results are expressed as a percentage of vehicle controls (mean ± SEM). **p < 0.01, # p < 0.05; n = 4–8. Scale bar = 100 μm