Fig. 5
From: Chronic spinal cord injury attenuates influenza virus-specific antiviral immunity
Spinal cord-injured mice produce significantly less virus-specific CD8+T cells than CT mice. a Splenocytes from naïve and SCI mice isolated 7-day post-infection were analyzed by flow cytometry using tetrameric complexes of either the nucleoprotein peptide (NP366–374) or the polymerase 2 protein peptide (PA224–233) presented by class I H-2Db (mean ± SEM, 4–5 mice/group, *p < 0.05). b, c In vitro intracellular cytokine assay revealed a significant decrease in the number of CD8+T cells producing IFNγ following stimulation with the peptide DbNP366 (b) and a significant decrease in the number of CD8+T cells producing IFNγ as well as both IFNγ and TNF following stimulation with the peptide DbPA224 (c). d Percentage of CD8+T cells expressing granzyme B, TNFα, and IFNγ following stimulation with peptide DbPA224. Bar graphs represent the mean ± SEM, n = 4–5 mice/group, *p < 0.05