Fig. 1
From: Complement system activation contributes to the ependymal damage induced by microbial neuraminidase
Detection of C5b-9 complex and C9 on the ependymal epithelium of rats injected with NA. a–d Immunohistochemistry using anti-C5b-9 showing the injected (a, c) and the contralateral (b, d) ventricles. Two hours after NA injection, a positive label could be observed on the ependymal surface of the injected ventricle (c), which was absent in the contralateral one (d). e–g Cryostat 10-μm sections double labeled with anti-C9 (green), anti-vimentin (red), and the nuclear stain DAPI (blue). The ependymal cells of the injected ventricle showed C9 deposits on the apical surface and cilia (e and g) that were not present in the contralateral ventricle (f). LV lateral ventricle, DAPI, 4,6-diamidine-2-phenylindole dihydrochloride, Ep ependyma, NP nervous parenchyma, CP choroid plexus, Str striatum, Sp septum. (Arrows) Damaged cells