Fig. 2
From: Remodeling of lipid bodies by docosahexaenoic acid in activated microglial cells
Lipid bodies observed in situ in adult mouse microglia. a Microglia (m) stained for IBA1 with immunoperoxidase that is observed in direct juxtaposition with two neurons (n). A protruding process contains a cellular inclusion (in) and a large LB resembling a droplet, in the hippocampus CA1 of an adult mouse (3Â months old). b Inset showing at higher magnification the cellular inclusion, which contains an accumulation of cellular membranes in the process of being digested and, the LB, which displays two electron densities suggesting different lipid compositions. c Microglial cell (m) stained for IBA1 with immunogold that contains several lipidic inclusions, among which a group of small lipid droplets (asterisk) juxtaposing two electron-dense profiles of lipofuscin granules (g), in the cerebral cortex of an adult mouse (3Â months). ma myelinated axon. d Inset showing at higher magnification the ultrastructural features and relationships between lipid vacuoles and lipofuscin granules. e Microglial process (m) stained for IBA1 with immunogold, observed in the same mouse, exhibiting several additional examples of lipidic inclusions. f Inset showing at higher magnification the inclusions: two profiles of lipofuscin granules surrounding an accumulation of very small lipid droplets (asterisk) grouped one to another. Different shades of gray can be noted among the lipid bodies, suggesting that they contain different lipid species. bv blood vessel