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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Remodeling of lipid bodies by docosahexaenoic acid in activated microglial cells

Fig. 7

DHA-induced changes in microglial cell phagocytosis. ab Effect of DHA on N9 mouse microglial phagocytic activity with and without LPS. N9 microglial cell were cultured for 6 h (a) in the presence or absence of DHA (50 μM) and LPS (100 ng/mL) or for 24 h (b) in the presence or absence of DHA (10, 25, 50 μM) and LPS (100 ng/mL). FluoSpheres (106 particles/mL) were added to the cells 3 h before the end of treatment. Cells were fixed using 4 % paraformaldehyde and labeled with Hoechst 33258 (10 μM, 10 min). Cells were mounted on glass slides and imaged under a fluorescent microscope. The number of FluoSpheres per cell was counted manually. Shown are average percentages of total cells containing FluoSpheres ± SEM from three independent experiments. *p < 0.01

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