Fig. 4

Co-expression analysis of macrophage polarization markers demonstrate incoherent expression in TBI macrophages. a Pairwise dot plots of absolute expression values of widely accepted signature macrophage polarization markers in TBI macrophages are shown. Co-expression analyses of signature genes within the same group were analyzed by linear regression analysis, and Pearson’s correlation coefficients (r ²) and the lines of best fit are presented. b Pairwise dot plot of absolute expression values of signature macrophage polarization markers across classes were analyzed in TBI macrophages. Results of linear regression analysis and r ² are shown. c Signature macrophage polarization markers were analyzed for their capacity to predict gene expression of other signature macrophage polarization markers and shown here in stacked dot plots. The capacity of Mrc1 (TPM > 1) or lack of Mrc1 (TPM = 0.01) in a TBI macrophage to predict the mean expression level of Il1b or Tnf was statistically analyzed by Mann-Whitney U tests. All p values were insignificant (p < 0.05). Similarly, the expression of or lack of expression of Socs2 in a TBI macrophage failed to predict the mean expression level of Tnf, Arg1, and Chi3l3