Fig. 6
From: Brain trauma elicits non-canonical macrophage activation states
RNA flow cytometry validates that TBI macrophages co-express macrophage polarization markers across classes in unusual combinations. RNA flow cytometry for M(LPS, IFNγ) markers, Tnf and Il1b, were analyzed for their co-expression with M(IL4) markers, Arg1, Mrc1, and Chi3l3, in ipsilateral TBI brain hemispheres 1 day post-TBI. Quadrant gates were drawn based on DapB RNA probe binding. N = 3 independent experiments, with pooled tissues of eight age-matched cage mate mice for each experiment