Fig. 2

Effects of KYC on IgG extravasation and neutrophil infiltration in the brain of MCAO mice. MCAO mice were treated with KYC (10 mg/kg/d) or PBS via i.p. starting 1 h after MCAO. Brain tissues were harvested 3 days after MCAO. a Gross photographs of endogenous IgG extravasation in the brain of MCAO mice. Brain coronal sections from rostral to caudal in 1-mm interval were immunostained using an anti-mouse IgG (brown); b Mean gray value of IgG exudation (*p < 0.05, KYC vs. PBS group, t test); c Neutrophil infiltration in brain cortex ischemic core. Frozen sections were immunostained with anti-NIMP-R14 antibody (marker for neutrophil); d Counts of NIMP-R14⁺ cells in the brain sections of mice 1, 3, and 7 days after MCAO; e Counts of NIMP-R14⁺/MPO⁺ cells in the brain sections of mice 1, 3, and 7 days after MCAO (n = 4/group, p < 0.05, KYC vs. PBS group, t test)