Fig. 4

Effects of KYC on apoptosis in the brain of mice 3 days after MCAO. MCAO mice were prepared as described in Fig. 2. a Images of TUNEL assay in the cortex ischemic core areas; b Images of p53 immunostaining in brain cortex ischemic core; blue: DAPI, red: p53, and purple: p53 + DAPI, as arrows indicated. c Counts of TUNEL positive cells (n = 5/group, *p < 0.05, KYC vs. PBS group, t test). d Counts of p53 positive cells (n = 4/group, *p < 0.05, KYC vs. PBS group, t test). e Western blot of nNOS in MCAO brains; f Density analysis of nNOS western blot. The data shown is the ratio of nNOS density to β-actin density. The data for sham group is normalized to 100 % (n = 6/group, one-way ANOVA with Bonferroni test, *p < 0.05 and **p < 0.01)