Fig. 5

Effects of KYC on MPO and ClTyr expression in the brain of mice 3 days after MCAO. MCAO mice were prepared as described in Fig. 2. a Western blot of MPO in brain tissues of MCAO mice; b Images of MPO immunostaining in cortex ischemic core areas; c ClTyr immunostaining in the cortex ischemic core areas; d Images of colocalization of MPO and ClTyr in brain cortex ischemic core; red: ClTyr, green: MPO, and yellow: ClTyr + MPO, as arrows indicated. All images here are the representative images of five repeat samples. e Counts of MPO positive cells (n = 5/group, *p < 0.05, KYC vs. PBS group, t test). f Counts of ClTyr positive cells (n = 5/group, *p < 0.05, KYC vs. PBS group, t test)