Fig. 6

Immunoblot analysis of the effects of FK506 on the FK506-binding protein, calcineurin, and markers of mitochondrial fusion in non-tg and gp120 tg mice. Non-tg and gp120 tg mice were treated with vehicle or FK506 for 4 weeks and were analyzed by immunoblot. a Representative Western blots are shown from the membrane fraction of whole brain lysate that was immunoreacted with antibodies against calcineurin, an FK506-binding protein, OPA1 and MFN1, GTPases that are required for mitochondrial fusion, and mtHSP70, a mitochondrial housekeeping control protein. b Computer-aided analysis of sections incubated with an antibody against calcineurin, which was unaffected by mouse type or treatment. c OPA1 was detected as a doublet which was non-significantly decreased in vehicle-treated gp120 tg mice compared to vehicle-treated non-tg mice. Treatment of gp120 mice with FK506 led to a statistically significant increase in OPA1 compared to vehicle-treated non-tg mice. d Levels of MFN1 were detected as a double tat approximately 70–80 kDa and were significantly increased in FK506-treated gp120 mice compared to vehicle-treated non-tg mice. e mtHSP70 was detected as a single band, was included as a control protein, and was unaffected by treatment or mouse type. *p < 0.05, by one-way ANOVA post hoc Dunnett’s; ^# p < 0.05, by one-way ANOVA post hoc Tukey-Kramer. N = 10; age = 12 months of age