Fig. 5

Activated P815 cells increased microglia activation. a The cells were stained with Iba1 antibody, and upregulated Iba1-immunopositive expression (green) on the activated microglia was observed using confocal scanning. The blue staining represents DAPI. Scale bar 50 μm was used to detect Iba1. b–d Expression levels of M1 phenotype markers (CD86, CD32, and IL-1β). e Expression levels of CD206, markers for M2a phenotype. f Expression levels of SOCS3, markers for M2b phenotype. ^* P < 0.05, ^** P < 0.01 vs. control group. ^# P < 0.05, ^## P < 0.01 vs. CM (P815 lps) group. Data are presented as the mean ± SD (n = 5)