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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Activated brain mast cells contribute to postoperative cognitive dysfunction by evoking microglia activation and neuronal apoptosis

Fig. 7

Activated P815 cells induced neuronal apoptosis directly. a Neuronal apoptosis was tested with TUNEL test. b The cells were stained with SYP and PSD95 antibody and upregulated SYP, and PSD95-immunopositive expression (green) on the activated microglia was observed using confocal scanning, respectively. The blue staining represents DAPI. Scale bar 50 μm was used to detect Iba1. c The expression of Bax, Bcl-2, SYP, and PSD95 was detected by Western blotting using specific antibodies in the hippocampus of rats. Each blot is representative of three experiments. d Expression of Bax was quantified and normalized to Bcl-2 levels. Expression of SYP and PSD95 were quantified and normalized to GAPDH levels. Each value was then expressed relative to the control, which was set to 1. * P < 0.05, ** P < 0.01 vs. control group. # P < 0.05, ## P < 0.01 vs. CM (P815 lps) group. Data are presented as the mean ± SD (n = 5)

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