Fig. 3
From: STIMs and Orai1 regulate cytokine production in spinal astrocytes
Orai1, STIM1, and STIM2 are important for SOCE in spinal astrocytes. a Effects of specific siRNAs against STIM1 or STIM2 on protein levels of STIM1 and STIM2 (n = 4–5 samples). b Effects of specific siRNAs against STIMs on TG-induced SOCE. Left panel, representatives of TG-induced calcium responses recorded in astrocytes transfected with control siRNA or targeting siRNAs against STIMs; right panel, summary of effects of control siRNA (n = 78), STIM1 siRNA (n = 77), and STIM2 siRNA (n = 69). c Effects of specific siRNAs against Orai1, Orai2, or Orai3 on protein levels of Orai1, Orai2, and Orai3 (n = 4 samples each). The protein levels were normalized to control (non-target siRNA). d Effects of specific siRNAs against Orais on TG-induced SOCE. Left panel, representatives of TG-induced calcium responses recorded in astrocytes transfected with control siRNA or targeting siRNAs against Orais; right panel, summary of effects of control siRNA (n = 112), Orai1 siRNA (n = 76), Orai2 siRNA (n = 40), or Orai3 siRNA (n = 42). Values represent mean ± SEM, *P < 0.05, compared with control by Student’s t test or the one-way ANOVA