Fig. 5From: STIMs and Orai1 regulate cytokine production in spinal astrocytesDepletion of calcium stores from endoplasmic reticulum induces STIM1-Orai1 puncta formation in spinal astrocytes. a Live-cell confocal images of astrocytes transfected with STIM1-YFP and Orai1-CFP taken before and after 10 min treatment of 1 μM thapsigargin (TG) or 30 μM cyclopiazonic acid (CPA). b Average number of STIM1 puncta (green) per 100 μm2 induced by TG or CPA. c Average number of STIM1/Orai1 puncta (yellow) per 100 μm2 induced by TG or CPA. Puncta were quantified as spots of high fluorescence intensity ranging from 0.4 to 2.0 μm in diameter size before and after 10 min TG and CPA treatments. Values represent mean ± SEM, *P < 0.05, compared with pretreatment by Student’s t testBack to article page