Fig. 2

Reduced disease severity but elevated viral loads in TMEV-infected P2/P3-Tg mice in the SJL background. a Development of clinical disease in TMEV-infected P2/3-Tg mice (n = 15) and their littermates (LM, n = 15) in the SJL background was examined for 120 days after infection with TMEV. Clinical symptoms were assessed weekly based on behavioral changes as described in the “Methods” section. No clinical symptoms were developed in the P2/P3-Tg mice in the B6 background (data not shown). b The titers of the infectious virus in the CNS of TMEV-infected P2/P3-Tg mice and their littermates were determined by plaque assay on the indicated number of days post-infection. The PFU numbers represent the PFUs per whole brain or spinal cord calculated from the pool of three to four mice per group. The statistical significance of the differences was analyzed by Student’s t test between control and Tg mice. *P < 0.05; **P < 0.01; and ***P < 0.001. d8, d21, and d120 represent day 8, day 21, and day 120 post-infection, respectively