Fig. 4

Antigen-specific IFN-γ-producing CD8⁺ T cell levels in the CNS of TMEV-infected P2/P3-Tg mice. a CNS-infiltrating cells from P2/P3-Tg mice and their littermates were isolated at 8, 21, and 120 days pi and stimulated with the indicated peptides (2 μM) for 6 h. Then, the cells were stained for both CD8 and intracellular IFN-γ, followed by flow cytometric analysis. The percentage of IFN-γ⁺ and CD8⁺ T cells is shown in the upper left quadrant of each plot. A representative flow cytometry plot from three independent experiments is shown. d8, d21, and d120 represent day 8, day 21, and day 120 post-TMEV infection, respectively. Tg and LM indicate P2/P3-Tg and littermate mice, respectively. b The proportions and the total numbers of epitope-specific IFN-γ-producing CD8⁺ T cells (left for B6 and right for SJL mice) are presented. c The proportions of D^b-VP2_121–130 tetramer-staining CD8⁺ T cells in the CNS of B6 mice and the proportions of K^s-VP3-_159–166 and VP3-_173–181 tetramer-positive CD8⁺ T cells in the CNS of SJL mice at 8 dpi are shown. Values given represent the mean percentages or numbers (±SD) of the results from three independent experiments. *P < 0.05; **P < 0.01; and ***P < 0.001