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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Transgenic expression of non-structural genes of Theiler’s virus suppresses initial viral replication and pathogenesis of demyelination

Fig. 6

Expression of viral and IL-6 genes in different cell types. a IL-6 expression and virus replication were tested in peritoneal macrophages from P2/P3-B6 mice. Peritoneal macrophages were infected with 1 or 5 MOI of TMEV for 18 h. The level of TMEV-VP1 reflecting viral replication was compared by real-time PCR. b IL-6 expression and virus replication in astrocytes were also tested after infection with TMEV (MOI of 2 and 10) for 18 h. (c) Antigen-presenting function of cells from control and Tg mice was tested based on the induction of T cell proliferation. Peritoneal macrophages or splenic CD11C+ DC cells were prepared from WT and P2/P3 Tg B6 mice. Positively purified splenic CD4+ cells from TMEV-infected B6 mice were cultured with macrophages or DCs as APCs for 48 h in the presence of PBS, CD4 peptides, UV-TMEV, or infectious TMEV, followed by 18 h of incubation with 1 μCu 3H-TdR. Values given are the means (±SD) of the results from triplicate cultures. *P < 0.05

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