Fig. 10

Abcc8−/− and Trpm4−/− protects against TLR4-mediated NOS2 induction in murine microglia. a, b Immunoblots (a) and quantification (b) for NFATc1 (90 kDa isoform shown), NOS2 (130 kDa), pCaMKII (α/β, 50/60 kDa), CaMKII (α/β, 50/60 kDa), pCN (60 kDa), and CN (60 kDa), for tissue homogenates from the striatum of wild-type (WT), Abcc8−/− and Trpm4−/− mice, 24 h after injection of aCSF (5 μL) or LPS (5 μL; 0.1 μg/μL) into the striatum; gels were run separately for each protein analyzed; quantitative data were normalized to a loading control for total protein, HSC70; three replicates; **p < 0.01. c Quantification of mRNA for microglial Cd11b, astrocytic Gfap, and neuronal Neun in freshly isolated microglia from mouse brain; data normalized to mRNA for Gapdh; three replicates. d Quantification of mRNA induction for Nos2 and Fth1 in primary cultured adult microglia from WT, Abcc8−/− and Trpm4−/− mice 24 h after exposure to LPS (1 μg/mL); data normalized to the response of LPS alone in WT microglia; three replicates; *p < 0.05; **p < 0.01