Fig. 2

Upregulation of Abcc8 and Trpm4 mRNA in TLR4-activated rat microglia in vivo. a–c Immunofluorescence labeling of P2Y12⁺ microglia (green) and fluorescence in situ hybridization (FISH) of mRNA transcripts of Abcc8, Trpm4, or Kcnj11 (red), following intrastriatal infusion (0.5 μL/h) of normal saline (NS) or LPS (5 μg/day) for 24 h (LPS); nuclei labeled with DAPI (blue); bar graphs quantification of microglial expression of Abcc8, Trpm4, or Kcnj11, expressed as fold change normalized to values with NS infusion; five replicates; **p < 0.01