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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia

Fig. 6

Sur1-Trpm4 is a negative regulator of SKF-96395-sensitive Ca2+ entry channels in murine N9 microglia. a Acute effect of LPS (1 μg/mL) on [Ca2+]i in N9 microglia, expressed as fluorescence (F) over baseline (F 0); data were obtained from ten cells per individual experiment; data shown are average responses of four independent replicates; the black arrow shows the time of LPS application. b Representative single cell traces of F/F 0 in control (CTR) and TLR4-activated (LPS 24 h) N9 microglia (left); also shown is the quantification of Ca2+ oscillations (right), expressed as the time series rolling standard deviation of F/F 0, in CTR cells and in TLR4-activated cells treated with vehicle (VEH), the Ca2+ entry antagonist, SKF-96395 (SKF; 7.5 μM), or the TLR4 signaling inhibitor, TAK-242 (3 μM); **p < 0.01. c, d Temporal changes in [Ca2+]i, expressed as F/F 0 (left panels), and magnitude of F/F 0 at the termination of recording (right panels), in control (c) and N9 microglia after TLR4-activation by LPS (1 μg/mL) for 24 h (d), following application of vehicle (Veh), the Sur1 antagonist, glibenclamide (Glib; 30 μM), the Sur1 agonist, diazoxide (Diaz; 100 μM), the Trpm4 antagonist or 9-phenanthrol (9Phe; 5 μM); also shown is the magnitude of F/F 0 after application of the Ca2+ entry antagonist, SKF-96395 (SKF; 7.5 μM) (right panels); the time of drug application was coincident with the start of recording; data were obtained from ten cells per individual experiment (left); average data collected at the end of 10 min recording from thee to five independent replicates are shown (right)

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