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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia

Fig. 7

Sur1-Trpm4 regulates NFATc1, CaMKII, and CN in murine N9 microglia. a, b Images (a) and quantification (b) of nuclear NFATc1 (white) in N9 microglia under control (CTR) conditions, following 15-min exposure to the Ca2+ ionophore, A23187 (1 μM), used as positive control, and following 24-h exposure to LPS alone (1 μg/mL), LPS plus glibenclamide (Glib; 30 μM), LPS plus 9-phenanthrol (9Phe; 5 μM), or LPS plus SKF-96395 (SKF; 7.5 μM); nuclei stained with DAPI (blue); typical nuclear diameter is 6–12 μm; quantitative data on specific nuclear labeling were normalized to the control; p < 0.01. c Immunoblot (left) and quantification of all bands (right) for NFATc1 in nuclear extracts from TLR4-activated N9 microglia treated with vehicle (Veh) or glibenclamide (Glib; 30 μM);quantitative data were normalized to a loading control for the nuclear protein, histone deacetylase 1 (HDAC); four replicates. d Immunoblot (left) and quantification of all bands (right) for phosphorylated CaMKII (pCaMKII), CaMKII, and phosphorylated CN (pCN) in whole cell lysate from TLR4-activated N9 microglia treated with vehicle (Veh) or glibenclamide (Glib; 30 μM); quantitative data were normalized to a loading control for total protein, HSC70; four replicates; *p < 0.05

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