Table 1 Primers used for qPCR in this study
From: The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia
Gene name | Species | Sequence accession number | Primer sequence | Amplicon length |
|---|---|---|---|---|
Abcc8 | Rat | NM_013039.2 | 5′-TCATCCGGGTGAGGAGATAC-3′ (+) | 130 |
5′-CACCAGTAGGTCCCCTTTGA-3′ (−) | ||||
Trpm4 | Rat | NM_001136229.1 | 5′-GCAAGTTCTGAGGACTCTGTTG-3′ (+) | 140 |
5′-TTGCATCCTGTTGCATGTTGGC-3′ (−) | ||||
Kcnj11 | Rat | NM_031358.3 | 5′-TGCGTCACAAGCATCCACTCCT-3′ (+) | 100a |
5′-GGACATTCCTCTGTCACCATGC-3′ (−) | ||||
Kcnj8 | Rat | NM_017099.4 | 5′-CACTTCGGGAGGTCTCTGC-3′ (+) | 69 |
5′-GCGTCCTCCTAGAAGACTCGG-3′ (−) | ||||
Il-1β | Rat | NM_031512.2 | 5′-AAATGCCTCGTGCTGTCTGA-3′ (+) | 85 |
5′-TGGAGAATACCACTTGTTGGC-3′ (−) | ||||
P2y12 | Rat | NM_022800.1 | 5′-CTTTGGCAACGAAACCAAGT-3′ (+) | 127 |
5′-CACCTCCATGGTCCTGGTTA-3′ (−) | ||||
Tlr4 | Rat | NM_019178.1 | 5′-TCATGCTTTCTCACGGCCTC-3′ (+) | 142 |
5′-AGGAAGTACCTCTATGCAGGGAT-3′ (−) | ||||
Gfap | Rat | NM_017009.2 | 5′-CCAGATCCGAGAAACCAGCC-3′ (+) | 88 |
5′-CCGCATCTCCACCGTCTTTA-3′ (−) | ||||
Neun | Rat | NM_001134498.2 | 5′-CGCAGCCTACAGTGACAGTTAT-3′ (+) | 132 |
5′-GTGAAGCGGCTGTACCCTC-3′ (−) | ||||
Gapdh | Rat | NM_017008.4 | 5′-CATCACTGCCACTCAGAAGACTG-3′ (+) | 153b |
5′-ATGCCAGTGAGCTTCCCGTTCAG-3′ (−) | ||||
Abcc8 | Mouse | NM_011510.3 | 5′-GCCAGCTCTTTGAGCATTGG-3′ (+) | 102 |
5′-AGGCCCTGAGACGGTTCTG-3′ (−) | ||||
Trpm4 | Mouse | NM_175130.4 | 5′-TGTTGCTCAACCTGCTCATC-3′ (+) | 83 |
5′-GCTGTGCCTTCCAGTAGAGG-3′ (−) | ||||
Kcnj11 | Mouse | NM_010602.3 | 5′-TGCGTCACAAGCATCCACTCCT-3′ (+) | 100c |
5′-GGACATTCCTCTGTCACCATGC-3′ (−) | ||||
Il-6 | Mouse | NM_031168.2 | 5′-CCCCAATTTCCAATGCTCTCC-3′ (+) | 141 |
5′-CGCACTAGGTTTGCCGAGTA-3′ (−) | ||||
Nos2 | Mouse | NM_010927.4 | 5′-TGGAGCGAGTTGTGGATTGTC-3′ (+) | 98 |
5′-GGGCAGCCTCTTGTCTTTGA-3′ (−) | ||||
Fth1 | Mouse | NM_010239.2 | 5′-CGAGATGATGTGGCTCTGAA-3′ (+) | 94 |
5′-TCTGCAGCTTCATCAGTTTCTC-3′ (−) | ||||
Cd11b | Mouse | NM_001082960.1 | 5′-AAGGATTCAGCAAGCCAGAA-3′ (+) | 100 |
5′-TACTCTTCAGAGCCCCATGC-3′ (−) | ||||
Gfap | Mouse | NM_001131020.1 | 5′-TGCTGGAGGGCGAAGAAAACCG-3′ (+) | 83 |
5′-TTTGGTGCTTTTGCCCCCTCGG-3′ (−) | ||||
Neun | Mouse | NM_001039167.1 | 5′-GTTGCCTACCGGGGTGCACAC-3′ (+) | 110 |
5′-TGCTCCAGTGCCGCTCCATAAG-3′ (−) | ||||
Rps18 | Mouse | NM_011296.2 | 5′-CGGAAAATAGCCTTCGCCATCAC-3′ (+) | 134 |
5′-ATCACTCGCTCCACCTCATCCT-3′ (−) | ||||
Gapdh | Mouse | NM_008084.3 | 5′-CATCACTGCCACCCAGAAGACTG-3′ (+) | 153d |
5′-ATGCCAGTGAGCTTCCCGTTCAG-3′ (−) |