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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Histamine induces microglia activation and dopaminergic neuronal toxicity via H1 receptor activation

Fig. 3

Histamine-induced microglial cytoskeleton rearrangement. a Representative confocal photomicrographs showing that 100 μM histamine (H100) induced membrane ruffling. Cells were stained for phalloidin (red), CD11b (green), and Hoechst 33342 (nuclei in blue). b Histamine also increased α-tubulin acetylation expression levels especially in some microglial processes (white arrows) as compared with the faint staining present in microtubule organization centers (asterisk) found in resting microglial cells, as detected by immunolabeling for acetylated α-tubulin (red), CD11b (green), and Hoechst 33342 (nuclei in blue). Scale bars 10 μm. c Bar graph displays the increased expression levels of acetylated α-tubulin in histamine-activated cells. Data are expressed as mean ± SEM (n = 4–8) and as a percentage of control. ***P < 0.001, using one-way ANOVA followed by Bonferroni’s multiple comparison test

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