Table 2 Antibodies and conditions
From: Microglial immunophenotype in dementia with Alzheimer’s pathology
Microglial protein | Species | Clone/company | Dilution | Antigen retrieval technique |
|---|---|---|---|---|
Iba1 | Rabbit | Polyclonal/Wako | 1:750 | Pressure cooker citrate pH 6 |
CD68 | Mouse | PG-M1/Dako | 1:50 | Microwave citrate pH 6 |
HLA-DR | Mouse | CR3/43/ThermoFisher Scientific | 1:200 | Microwave citrate pH 6 |
MSR-A | Goat | Polyclonal/R&D Systems | 1:500 | Microwave citrate pH 6 |
CD64 | Goat | Polyclonal/R&D Systems | 1:100 | Microwave EDTA pH 8 |