Fig. 1From: Reduction of cytosolic phospholipase A2α upregulation delays the onset of symptoms in SOD1G93A mouse model of amyotrophic lateral sclerosiscPLA2α protein levels in the spinal cord of hmSOD1 mice during the development of the disease in hmSOD1 mice. a Representative cPLA2α protein expression in the spinal cord tissue sections along the hmSOD1 mouse life span. Scale bars = 250 μm for upper section and 50 μm for lower sections. The mean ± SEM of the cPLA2α fluorescence intensity is presented in the bar graph (n = 8 mice for each time point, five fields were analyzed for each mouse). *p < 0.001—significance from WT mice. b, c Development of the disease monitored by Kaplan-Meier survival curve and motor performance on accelerating rotarod test (n = 12 mice in each group). d Representative spinal cord sections of neurons (NeuN), microglia (Iba-1 and CD40), and astrocytes (GFAP) during the hmSOD1 mouse life span. The mean ± SEM of percentage of the cell area is presented in the bar graphs (n = 8 for each time point, five fields were analyzed for each mouse). Scale bars = 50 μm and in the inset 20 μm. *p < 0.001—significance from WT mice. e Representative staining of peripheral monocytes detected by double staining of Iba-1 and CD169, shown by arrows. Scale bars = 250 μm for left section and 50 μm for right section in each pair. Scale bars in the insets = 20 μm. The mean ± SEM of number of monocytes and of percentage of the cell area is presented in the bar graphs (n = 8 for each time point, five fields were analyzed for each mouse). *p < 0.001—significance in comparison to week 19Back to article page