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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Reduction of cytosolic phospholipase A2α upregulation delays the onset of symptoms in SOD1G93A mouse model of amyotrophic lateral sclerosis

Fig. 1

cPLA2α protein levels in the spinal cord of hmSOD1 mice during the development of the disease in hmSOD1 mice. a Representative cPLA2α protein expression in the spinal cord tissue sections along the hmSOD1 mouse life span. Scale bars = 250 μm for upper section and 50 μm for lower sections. The mean ± SEM of the cPLA2α fluorescence intensity is presented in the bar graph (n = 8 mice for each time point, five fields were analyzed for each mouse). *p < 0.001—significance from WT mice. b, c Development of the disease monitored by Kaplan-Meier survival curve and motor performance on accelerating rotarod test (n = 12 mice in each group). d Representative spinal cord sections of neurons (NeuN), microglia (Iba-1 and CD40), and astrocytes (GFAP) during the hmSOD1 mouse life span. The mean ± SEM of percentage of the cell area is presented in the bar graphs (n = 8 for each time point, five fields were analyzed for each mouse). Scale bars = 50 μm and in the inset 20 μm. *p < 0.001—significance from WT mice. e Representative staining of peripheral monocytes detected by double staining of Iba-1 and CD169, shown by arrows. Scale bars = 250 μm for left section and 50 μm for right section in each pair. Scale bars in the insets = 20 μm. The mean ± SEM of number of monocytes and of percentage of the cell area is presented in the bar graphs (n = 8 for each time point, five fields were analyzed for each mouse). *p < 0.001—significance in comparison to week 19

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