Fig. 2From: Reduction of cytosolic phospholipase A2α upregulation delays the onset of symptoms in SOD1G93A mouse model of amyotrophic lateral sclerosisElevated cPLA2α protein expression in the different cell types of the spinal cord. a Representative pictures of immunofluorescence double staining of cPLA2α (green) with motor neurons (NeuN), microglia (Iba-1), or astrocytes (GFAP) in the spinal cord during hmSOD1 mouse life span (out of 8 mice in each time point, presented in Fig. 1a). DAPI staining for cell nuclei. Scale bars = 20 μm. b Insets of the immunofluorescence double staining of cPLA2α with motor neurons, microglia, or astrocytes (presented in a), in a higher magnification. Scale bars = 20 μm. c Immunofluorescence double staining of phospho-cPLA2α (p-cPLA2) and motor neurons (NeuN), microglia (Iba-1) and astrocytes (GFAP). Shown representative immunofluorescence confocal microscopy pictures (out of 8 mice in each group). Lower panel presents the cell staining and DAPI to show the cell nuclei. Scale bars = 20 μmBack to article page