Fig. 5
ELISA of IL-1β and IL-3 cytokines released from MOG35-55-stimulated TMOG cells in the presence or absence of CBD. APC/TMOG were stimulated with MOG35-55 at 5 μg/ml for 24 h in the presence or absence of CBD at 5 μM and the amounts of (a) IL-1β and (b) IL-3 in the culture media determined. 100 % is equivalent to maximal MOG35-55 effect which refers to 15 ± 6 pg/ml for IL-1β and to 377 ± 100 pg/ml for IL-3. Statistics (one way ANOVA) and symbols: a F(3,6) = 6.7, p < 0.05. b F(3,15) = 80.25, p < 0.001; Symbols: # p < 0.05, ### p < 0.01 vs Control, *p < 0.05, ***p < 0.001 vs MOG-35-55-stimulated cells