Skip to main content

Advertisement

Table 6 qPCR validation of various transcripts in mRNA prepared from MOG35-55-stimulated TMOG cells and the effect of CBD

From: Pathways and gene networks mediating the regulatory effects of cannabidiol, a nonpsychoactive cannabinoid, in autoimmune T cells

Symbol Gene name MOG alone [fold] $ CBD+MOG [fold] $ CBD alone [fold] $ One-way ANOVA
Xcl1 Chemokine (C Motif) Ligand 1 62.7 ± 0.7## 17.9 ± 11.4** 1.6 ± 0.1 F(3,8) = 12.6, p < 0.01
IL12a Interleukin 12 4.3 ± 0.1# 2.6 ± 0.3* 0.8 ± 0.1 F(3,8) = 6.0, p < 0.05
Btla B- and T-lymphocyte attenuator 2.8 ± 0.4## 7.1 ± 0.4*** 6.0 ± 0.5### F(3,8) = 60.9, p < 0.001
Dusp6 Dual specificity phosphatase 6 2.4 ± 0.4 3.3 ± 0.7# 1.9 ± 0.3 F(3,8) = 3.3, p = 0.07
Irf4 Interferon regulatory factor 4 3.4 ± 0.6## 7.9 ± 0.5*** 3.7 ± 0.3## F(3,8) = 43.3, p < 0.001
Lag3 & Lymphocyte-activation gene 3 11.5 ± 0.3### 25.9 ± 2.3*** 5.8 ± 1.5* F(3,8) = 114, p < 0.001
IL10 & Interleukin 10 1.5 ± 0.3 3.4 ± 0.8# 1.4 ± 0.3 F(3,11) = 3.6, p = 0.05
  1. $Data are expressed as fold change vs control, not stimulated cells (equal to 1). # p < 0.05, ## p < 0.01, ### p < 0.01 vs control TMOG cells. *p < 0.05, **p < 0.01, ***p < 0.001 vs MOG-35-55-stimulated TMOG cells. &results reported previously [25]