Pathways and gene networks mediating the regulatory effects of cannabidiol, a nonpsychoactive cannabinoid, in autoimmune T cells

Table 6 qPCR validation of various transcripts in mRNA prepared from MOG35-55-stimulated T_MOG cells and the effect of CBD

Symbol	Gene name	MOG alone [fold] ^$	CBD+MOG [fold] ^$	CBD alone [fold] ^$	One-way ANOVA
Xcl1	Chemokine (C Motif) Ligand 1	62.7 ± 0.7^##	17.9 ± 11.4**	1.6 ± 0.1	F(3,8) = 12.6, p < 0.01
IL12a	Interleukin 12	4.3 ± 0.1^#	2.6 ± 0.3*	0.8 ± 0.1	F(3,8) = 6.0, p < 0.05
Btla	B- and T-lymphocyte attenuator	2.8 ± 0.4^##	7.1 ± 0.4***	6.0 ± 0.5^###	F(3,8) = 60.9, p < 0.001
Dusp6	Dual specificity phosphatase 6	2.4 ± 0.4	3.3 ± 0.7^#	1.9 ± 0.3	F(3,8) = 3.3, p = 0.07
Irf4	Interferon regulatory factor 4	3.4 ± 0.6^##	7.9 ± 0.5***	3.7 ± 0.3^##	F(3,8) = 43.3, p < 0.001
Lag3 ^&	Lymphocyte-activation gene 3	11.5 ± 0.3^###	25.9 ± 2.3***	5.8 ± 1.5*	F(3,8) = 114, p < 0.001
IL10 ^&	Interleukin 10	1.5 ± 0.3	3.4 ± 0.8^#	1.4 ± 0.3	F(3,11) = 3.6, p = 0.05

^$Data are expressed as fold change vs control, not stimulated cells (equal to 1). ^# p < 0.05, ^## p < 0.01, ^### p < 0.01 vs control T_MOG cells. *p < 0.05, **p < 0.01, ***p < 0.001 vs MOG-35-55-stimulated T_MOG cells. ^&results reported previously [25]

ISSN: 1742-2094