Fig. 5

Activation status of IL-1β⁺ cells in microglia nodules. Microglia nodules were classified as clusters of MHC class II⁺ cells in otherwise NAWM. These clusters of activated microglia were double stained for IL-1β (in brown) and cell surface markers associated with pro-inflammatory or anti-inflammatory cellular phenotypes (in red). IL-1β staining colocalized with MHC class II (a) and cell surface markers CD74 (b) and CD40 (c) as well as with CD200R (d). In most microglia nodules, IL-1β staining also colocalized with CCL22 (e), although some microglia nodules contained IL-1β⁺/CCL22⁻ cells. IL-1β⁺ microglia did not express MR (f). Original magnifications: ×40, scale bar represents 50 μm. Nuclei were counterstained with hematoxylin (blue)