Fig. 3

Effect of inhibition or deficiency of Nox2 activity on the function and viability of dopaminergic neurons after exposure to WT or mutated A29-V40 peptides or LPS. a Changes in the ability of rat neuron-glia cultures to take up ³H-labeled DA after exposure to WT α-Syn peptide A29-V40, its corresponding mutant A29-V40 (A30P), or LPS in the presence or absence of the Nox2 inhibitor apocynin (Apo). b Changes in the number of TH-positive neurons in rat neuron-glia cultures after exposure to WT α-Syn peptide A29-V40, its corresponding mutant A29-V40 (A30P), or LPS in the presence or absence of Apo. c Changes in the ability of WT or gp91^phox−/− mouse neuron-glia cultures to take up ³H-labeled DA after exposure to WT α-Syn peptide A29-V40 and its corresponding mutant A29-V40 (A30P). d Changes in the number of TH-positive neurons in WT or gp91^phox−/− mouse neuron-glia cultures after exposure to WT α-Syn peptide A29-V40 and its corresponding mutant A29-V40 (A30P). In a, b, two-way ANOVA and Bonferroni’s post hoc test were performed. n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the corresponding DMSO-treated controls; ^# P < 0.05 and ^## P < 0.01, compared as indicated. In c, d, one-way ANOVA and Dunnett’s test were performed. n = 5; *P < 0.05, **P < 0.01, compared with the corresponding basal controls