Fig. 8

Effect of CTB on Treg cells, γδT cells, IL-17-producing γδT cells, and Th17 cells. a Treg cells in the peripheral blood of the CTB-sham group were significantly increased both at 24 and 72 h after ischemia, compared with those of the sham group. CTB dose-dependently increased Treg cells in the peripheral blood both at 24 and 72 h after ischemia. b Treg cells in the sham ischemic hemisphere of the CTB-sham group both at 24 and 72 h after ischemia were not significantly different from those in the sham ischemic hemisphere of the sham group. In the ischemic hemisphere, CTB did not alter the percentage of Treg cells at 24 h, but a dose-dependent increase was found at 72 h after ischemia. c γδT cells in the peripheral blood of CTB-sham group both at 24 and 72 h after ischemia were not significantly different from those of the sham group. CTB dose-dependently suppressed the increase of γδT cells at 24 h, while it did not affect the proportion of γδT cells at 72 h after ischemia in the peripheral blood. d γδT cells in the sham ischemic hemisphere of the CTB-sham group both at 24 and 72 h after ischemia were not significantly different from those in the sham ischemic hemisphere of the sham group. In the ischemic hemisphere, the increases of γδT cells at 24 and 72 h after ischemia were dose-dependently suppressed by CTB. e In the ischemic hemisphere, the increases of IL-17-producing γδT cells at 24 and 72 h after ischemia were dose-dependently suppressed by CTB. f In the ischemic hemisphere, CTB had no effect on the Th17 cells at 24 and 72 h after ischemia. Bars represent mean ± SD (n = 8); *p < 0.05 vs MCAO group, #p < 0.05 vs CTB-L group, and &p < 0.05 vs SHAM group