Fig. 3

The expression of key regulators of the classical and alternative complement pathways are unchanged in MS cortical grey matter. Complement C1-inhibitor+ (C1INH, classical pathway regulator), factor H+ (FH; alternative pathway regulator) and clusterin (Clu, terminal pathway regulator) expression in and on cells of the cortical grey matter (a–c′; cells with a distinct neuronal morphology are shown in insets and arrows indicate cytoplasm/membrane immunoreactivity). The number of C1INH+ (d) and FH+ (e) cells were unchanged in GMLs in comparison to controls. There was an increase in the density of Clu+ cells (f) in the GMN of the deep cortical laminae and in the GMN and GMLs of the subpia. Each data point represents the mean value per area of interest and group means and 95 % confidence interval are shown. Groups compared with Kruskal-Wallis and Dunn’s multiple comparison post-test. Scale bars: a–c, 100 μm