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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity

Fig. 2

OSM inhibits d-aspartate uptake in primary mouse cortical astrocytes. a Shows saturation isotherm of Na+-dependent 3H-d-aspartate uptake by astrocytes. Primary astrocytes from wild-type (C57BL/6J) mouse neonatal (P2) brains were incubated with 3H-d-aspartate (0 to 200 μM) dissolved either in a Na+ buffer or in a Na+-free buffer (where Na+ is replaced by N-methyl-d-glucamine chloride, NMG, to determine nonspecific tritium retention) for 10 min at room temperature. Each point represents the mean ± SEM of at least three separate experiments, measured in triplicate. Kinetic constants were determined by nonlinear regression fit or a rectangular hyperbola, where V max was 0.3 μM ± 0.08 and K M was 22.4 μM (95 % confidence interval: 0.17–0.34 μM). b Shows the effect of OSM treatment (1 and 10 ng/mL; for 24 h) on 3H-d-aspartate uptake in cortical astrocytes culture. Data are normalized to untreated controls and presented as mean ± SEM; *p < 0.05, **p < 0.01, n = 3 to 17; one-way ANOVA followed by Dunnett’s multiple comparison test

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