Fig. 6From: Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicityEcoHIV induces OSM release and gp130 mRNA, but not OSMR-β mRNA or protein, in cultured primary mouse microglia. a Shows real-time PCR analysis of HIV LTR mRNA in control and EcoHIV-infected (35,000 pg of p24, for 24 h) primary microglia. ****p < 0.0001, n = 3. b Shows ELISA analysis of secreted OSM proteins in culture supernatants collected from control and EcoHIV-infected primary microglia. *p < 0.05, n = 6. c Shows real-time PCR analysis of gp130 and OSMR-β mRNA in control and EcoHIV-infected primary microglia. *p < 0.05, n = 3. d Shows Western blot analysis for OSMR-β proteins in control and EcoHIV-infected primary microglial cell lysates obtained from three independent experiments. β-Actin served as the loading controlBack to article page