Fig. 7

EcoHIV neither induces OSM secretion, nor the expression of gp130 or GLAST mRNA, but stimulates the expression of GLT-1 and OSMR-β in primary cortical astrocytes. a Shows real-time PCR analyses of HIV LTR mRNA in control and EcoHIV-infected (35,000 pg of p24, for 24 h) cortical astrocyte cultures. ****p < 0.0001, n = 3. b Shows ELISA analysis of secreted OSM proteins in culture supernatants collected from control and EcoHIV-infected primary astrocyte cultures. n = 6. c, d Shows real-time PCR analyses of GLAST and GLT-1 (c) and gp130 and OSMR-β (d) mRNA in control and EcoHIV-infected cortical astrocyte cultures. *p < 0.05, **p < 0.01, n = 3. e Shows Western blot analyses for OSMR-β proteins in control and EcoHIV-infected cortical astrocyte culture lysates. The left panel shows representative blot of three independent experiments. The right panel shows densitometric analysis of OSMR-β proteins. Data are presented as percentage of each respective ratio between optical density value of OSMR-β band (110 kDa) intensity and optical density value of the matched β-actin (42 kDa; loading control) band intensity. *p = 0.027, n = 3; two-tailed Student’s t test