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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Phenotypic clustering: a novel method for microglial morphology analysis

Fig. 1

The characterization of microglial cells by morphological criteria. a Confocal images, representing a sub-part of the analysed image in the frontal cortex region after maximum intensity projection. The individual microglia based on GFP fluorescence appears in white outline. The scale bar equals 50 μm. b Ramification detection based on GFP fluorescence with AcapellaTM software. The segmented ramifications linked to an individual microglia are shown artificially in green, the unattributed ramifications in white. The scale bar equals 50 μm. c The morphological criteria to characterize a microglial cell. The cell body detection (blue) and cytoplasm area (pink) have performed as a starting point to characterize a microglial cell. The complexity index (green) and the covered environment area (CEA in orange) have been deduced from ramification detection. The scale bar equals 10 μm. d Two-dimensional cartography at a single cell resolution. Colours correspond to the range of complexity and CEA with a gradient from a low level of complexity and CEA (yellow) to a high level of complexity and CEA (red). The scale bar equals 50 μm. For illustration, the images are contrast adjusted to aid in visualizing the GFP expression

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