Fig. 5

Effects of RBE on the activation of JNK and degradation of IkB-α induced by LPS. Cells were pre-treated with RBE (50–300 μg/ml) for 24 h followed by stimulation with or without LPS (10 ng/ml) for 30 min (for phospho-JNK) and 15 min (for IkB-α). Whole cell lysates were subjected to western blots analyses. Representative blots (upper panel) and densitometry analyses (lower panel) are shown a pJNK and b IkB-α. Statistical analyses were carried out by using one-way ANOVA with post hoc Student-Newman-Keuls test (multiple comparisons). Results are expressed as means ± SE of three three to four independent experiments. ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001 compared with LPS (10 ng/ml)-activated cells or control cells (for IkB-α graph)