Fig. 3

No changes in microglial activation or cytokine and chemokine protein expression in the eyes 6 h after temporal SE. No changes were observed in number of Iba1+ cells (a) or in Iba1+ cell morphology (b) between NSC and SE. Representative Western blot and quantification of CX3CR1 protein (~50 kDa) relative to NSC in the ipsilateral and contralateral eye 6 h post SE (c). Quantification of cytokine and chemokine protein expression, using mesoscale multiplex ELISA, in ipsilateral (d) and contralateral (e) eye homogenates 6 h after SE showed no differences compared to NSC. Data are presented as mean ± SEM, n = 5 NSC and n = 4 SE for WB and ELISA, n = 4 NSC and n = 4 SE for cell count and morphology. *p ≤ 0.05, unpaired t test in (a) and (c–e) two-way ANOVA in (b)