Fig. 1

Microglial/macrophage activation and ICH. Brain sections were immunostained for Iba1 (a) as described in the “Methods” section. After counter staining the sections with DAPI, the staining was analyzed using confocal microscopy. ICH remarkably induced microglial/macrophage activation as evidenced by cellular hypertrophy with enhanced Iba1 staining. Scale bar = 20 μm n = 3–6/group. b The average number of Iba1-positive cells per 0.1 mm² in the ipsilateral striatum. c The fluorescence intensity quantification of Iba1 immunoreactivity using ImageJ (NIH, USA). *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham