Fig. 3
From: Interleukin-1β has trophic effects in microglia and its release is mediated by P2X7R pore
a–c Electron micrographs of microglia from cultures expressing P2X7R-EGFP. As can be noted within the nodular structures, there resides small vesicles ranging in size from 100 to 200 μm (arrows) (a) and there is expression of P2X7R within the membrane of these vesicles (b, c). Scale bar 1 μm. d The vesicular structures expressing P2X7R were co-localized with expression of IL-1β. Green: anti-P2X7R antibody followed by Alexa Fluor 488. Red: anti-IL-1β antibody with secondary anti-Texa Red X. Far right image shows co-localization of P2X7R and IL-1β. e–f the vesicular structures expressing P2X7R also expressed LAMP-1 (marker of lysosomal vesicles). Green: anti P2X7R antibody followed by Alexa Fluor 488. Red: LAMP 1 antibody with secondary anti-Texa Red X. Far right image shows co-localization of IL-1β in lysosomes