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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Interleukin-1β has trophic effects in microglia and its release is mediated by P2X7R pore

Fig. 5

a An activated microglia expressing P2X7R-EGFP, subsequently loaded with FM1-43. When this cell was then stimulated with BzATP (100 μM), vesicles of ~200-μm diameter appeared on the surface of the cell which was followed by subsequent “release events” (see Additional file 1 for detailed images of the experiment in real time). The exocytosis was relatively fast to begin with, starting within 15 s of application of the agonist, reaching its maximum within 90–120 s and continuing for a duration of 5 min. b Change in membrane fluorescence as a measure of vesicular exocytosis in FM-143-loaded cells in response to 100 μM BzATP. Change in membrane fluorescence from cells expression wild-type P2X7R (P2X7R-EGF; red line) and loaded with FM-143 as compared to those expressing the non-pore-forming mutant (P2X7RG345Y-EGFP; blue lines) as compared to those expressing wild-type P2X7R (P2X7R-EGF) but pre-treated with oxATp (purple lines) (100 μM). c The sum of exocytosis was presented as change in membrane fluorescence. In response to 100 μM BzATP, there was a significantly higher amount of vesicular exocytosis from microglia expressing the pore-forming wild-type P2X7R (P2X7R-EGF) as compared to those expressing the non-pore-forming mutant (P2X7RG345Y-EGFP) or those expressing the wild-type P2X7R with prior inhibition with P2X7R antagonist, oxATP (100 μM)

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