Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Interleukin-1β has trophic effects in microglia and its release is mediated by P2X7R pore

Fig. 6

Media derived from primary hippocampal cultures expressing the pore-forming P2X7R contain a factor trophic for microglia. a Representative image of untransfected primary hippocampal cultures exposed for 72 h to the supernatant derived from cultures expressing P2X7R-EGFP. Red, isolectin GS-IB4; blue, DAPI nuclear stain. Scale bar, 10 μm. b The percentage of microglia (isolectin GS-IB4-positive cells) over the total number of cells (DAPI-positive cells) per imaging field was increased in cultures exposed to P2X7R-EGFP-derived supernatant compared to P2X7RG345Y-EGFP supernatant. c The absolute number of microglia (isolectin GS-IB4-positive cells) per imaging field was increased in cultures exposed to P2X7R-EGFP-derived supernatant compared with P2X7RG345Y-EGFP supernatant. Cell numbers were determined from 49 and 48 randomly selected fields (xy dimensions: 230 × 230 μm), respectively, from at least three independent experiments, 72 h post exposure to the supernatants. Data are mean ± SEM. d ELISA for IL-1β shows significantly higher levels of the proinflammatory cytokine release in primary hippocampal cultures expressing P2X7R-EGFP (n = 6) than in those expressing P2X7RG345Y-EGFP (n = 6). The absorbance data were measured in duplicates. The results show mean ± SEM. *p < 0.05 (Student’s t test)

Back to article page