Fig. 6

Glial (GFAP+) and neuronal nuclei (NeuN+) activity after MCAO. Double immunostaining of GFAP/NeuN at edges of lesion core of control (a), day 5 (b), day 7 (c), day 11 (d), and day 18 (e). The corresponding right panels present ×20 magnification images of GFAP and NeuN/DAPI. The “photomerged” image shows the entire focus of GFAP/NeuN in STR of the ipsilateral hemisphere (f). Quantification of GFAP fluorescence intensity in the ischemic hemisphere over reperfusion courses (g). ^* P < 0.05 versus day 11 and the control. ^** P < 0.01 versus day 18 and control, day 5, day 7. Quantification of NeuN fluorescence intensity in the same area of GFAP over reperfusion courses (h). ^** P < 0.01 versus day 5 and control. ^** P < 0.01 versus day 7 and control. ^** P < 0.01 versus day 11 and control. ^*** P < 0.001 versus day 18 and control. ^** P < 0.01 versus day 18 and day 5. ^* P < 0.05 versus day 18 and day 7, day 11. Scale bars = 50 and 20 μm