Fig. 3

DcR3 treatment reduced LPS-induced NO production and iNOS expression in the primary microglia culture. a The production of NO was determined by measuring the nitrite amount produced by microglial cells after LPS stimulation. The NO production was elevated in microglial cells after LPS stimulation. In contrast, DcR3.Fc-treated microglia had significantly reduced NO production after LPS stimulation (***p < 0.001, N = 3). b Immunofluorescence staining of iNOS- and DAPI-positive cells in LPS-stimulated microglia with (lower left) or without (upper left) DcR3.Fc treatment. The average of the integrated optical density (IOD) of iNOS relative to the total cells (DAPI+ cells) in three or four random fields in each well was shown reduced in the DcR3.Fc-treated group (right, **p < 0.01, ***p < 0.001, N = 3). The results are presented as means ± SEM